CH200 medium

CH200 Medium is specifically formulated for the optimal growth of Chinese Hamster Ovary (CHO) cells, including CHOS, CHO-K1, DG44, and DXB11 cell lines. This medium is ideal for enhancing recombinant protein production and supporting transfection assays in serum-free suspension cultures.

CH200 is a chemically defined, serum-free, protein-free, and animal origin-free medium, ensuring consistent performance with no variability from undefined components such as hydrolysates or animal-derived ingredients.

Key Features:

• Optimized for CHO Cells: Supports robust growth of CHOS, CHO-K1, DG44, and DXB11 lines
• High Recombinant Protein Production: Enhances yields in protein expression systems
• Chemically Defined Formula: No proteins, hydrolysates, or undefined components for consistent batch-to-batch results
• Serum-Free & Animal Origin-Free: Reduces risk of contamination and simplifies regulatory compliance
• Versatile Applications: Suitable for suspension cultures, recombinant protein production, and transfection assays

Culture Conditions:

• Cell Line Compatibility: CHO cells (CHOS, CHO-K1, DG44, DXB11)
• Culture Type: Suspension
• Culture Vessels: Flasks, plates, dishes, or culture bags
• Incubation Conditions:
  • Atmosphere: 5–8% CO₂ in a humidified environment
  • Temperature: 36℃ to 38℃
  • Shaker Speed (for suspension cultures): 120–130 rpm

Storage & Handling:

• Storage Temperature: 2℃ to 8℃
• Light Protection: Store in a dark environment to maintain medium stability

Applications:

• Recombinant Protein Production: Optimized nutrient composition for high-yield expression
• Transfection Assays: Supports efficient gene transfer and expression in CHO cells
• Suspension Cell Culture: Designed for scalable bioproduction systems