Digital PCR - QIAcuity One, 5Plex

QIAcuity Digital PCR Systems

The QIAcuity systems provide a powerful digital PCR (dPCR) approach for precise absolute quantification of target DNA or RNA. This method eliminates the need for standard curves and offers reliable quantification, mutation detection, genome editing analysis, and gene expression studies, among other applications.

Key Features:

• Absolute Quantification: Accurately determines the absolute amount of target DNA or RNA without the need for standard curves.
• Mutation Detection: Allows for sensitive detection of genetic mutations with high accuracy.
• Genome Editing Analysis: Ideal for studying genome modifications and the effects of gene editing technologies.
• Copy Number Variation (CNV): Facilitates the analysis of variations in gene copy numbers.
• Gene Expression Analysis: Enables precise quantification of gene expression levels.

System Capabilities:

• End-Point PCR: Works with end-point PCR to quantify target molecules.
• Single Partition PCR Reaction: PCR reactions occur in numerous single partitions, increasing the precision of results.
• Ideal Amplicon Size: Works best with amplicons in the range of 60–150 bp.
• Target Detection: Targets are detected through fluorescence, using either sequence-specific DNA probes or intercalating dyes.
• Partition Filling Detection: A passive fluorescent dye in the reaction mix identifies the total number of filled partitions, further aiding in accurate quantification.

Workflow:

1. Partitioning: The sample is partitioned into many individual reactions.
2. Thermocycling: PCR amplification takes place within each partition.
3. Imaging: The partitions are imaged to measure fluorescence and determine target quantities.

Technology:

• Nanoplate-Based System: Utilizes nanoplate technology for efficient partitioning and analysis, enabling high-throughput, accurate results.