Digital PCR - QIAcuity One, 5Plex

QIAcuity systems are designed to determine absolute amounts of target DNA or RNA in a sample by using a digital PCR approach. Ø absolute quantification Ø mutation detection Ø genome editing analysis Ø copy number variation (CNV) Ø gene expression analysis • End-point PCR • PCR reaction in many single partitions • Amplicon sizes with an ideal range of 60bp-150bp • Target molecule is detected by measuring the fluorescence o sequence specific DNA probes o or intercalating dyes • Total number of filled partitions is identified by a passive fluorescent dye, present in the reaction mix • Absolute quantification by dPCR eliminates the need of standard curves to determine amounts of target DNA in a given sample. • Nanoplate-based system • Workflow o Partitioning o Thermocycling o Imaging