Cytiva Sephadex™ G-25 Media

Cytiva™ Sephadex™ G-25 Media

Application

Gel filtration medium for desalting, buffer exchange, and contaminant removal in protein purification workflows. Ideal for biopharmaceutical production, research labs, and industrial-scale processes requiring rapid sample preparation.

Product Details

• Product Code: 10669455
• Brand: Cytiva 17-0033-02
• Packaging: 500 g lab pack
• Grades: Coarse, Medium, Fine, Superfine

Key Features

• Robust performance: Works across pH 2-13 for compatibility with harsh buffers
• Scalable design: Available as loose resins or prepacked columns for lab-to-production use
• High flow rates: >100 cm/h operational flow under recommended pressures
• Rigid bead structure: Spherical dextran particles maintain bed integrity during processing

Technical Specifications

• Particle size: 38-235 μm (wet form)
• Bed volume: 4-6 mL/g in distilled water
• Fractionation range: 1×10³ to 5×10³ M_r globular proteins
• Storage: Dry resins at 4-30°C; used resins in 20% ethanol/0.01M NaOH at 4-8°C

Operational Guidelines

• Follow Darcy's Law for pressure-flow relationships
• Column height recommendations: 30 cm bed height for optimal resolution
• Compatible with automated chromatography systems