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Product Details

Manufacturer
Cytiva
SKU
15239824

About this product

Amersham™ Protran® NC Nitrocellulose Membranes (Sheets, 8/pkg)

Premium-quality transfer membranes designed for superior protein binding—trusted by researchers worldwide for consistent, high-performance Western blotting and other protein analysis applications.

Application: Ideal for protein blotting, dot/slot blot assays, and nucleic acid transfer in molecular biology, biochemistry, and clinical research.

Product Details:

  • Material: Pure nitrocellulose

  • Pore Size: 0.45 µm (optimal for proteins >20 kDa)

  • Dimensions: 20 × 20 cm sheets

  • Package: 8 sheets/pack

Key Features:

  • Exceptional Protein Binding:

    • High binding capacity (80–100 µg/cm² for most proteins)

    • Low background noise

  • Enhanced Performance:

    • Fast wetting (≤15 seconds)

    • Compatible with colorimetric, chemiluminescent, and fluorescent detection methods

  • Reliable Quality:

    • Batch-to-batch consistency

    • No additives or surfactants

Technical Specifications:

  • Protein retention: >99%

  • Typical binding: BSA ≈ 90 µg/cm²

  • Temperature stability: -20°C to +60°C

Recommended Protocols:

  • Block with 5% non-fat dry milk or BSA

  • Use with standard transfer buffers (e.g., Towbin buffer)

  • Store at room temperature with desiccant

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