Flow Cytometry

Flow cytometry is a powerful, high-throughput analytical technique used to identify, characterize, and sort cells or particles based on their physical and chemical properties. Widely employed in immunology, oncology, stem cell research, microbiology, clinical diagnostics, and drug discovery, flow cytometry enables researchers to analyze multiple parameters from thousands of cells per second with exceptional speed and precision. In flow cytometry, cells are suspended in a fluid and passed through a laser beam one by one. As the cells interact with the light, they scatter and emit fluorescence if labeled with specific antibodies or dyes. These signals are detected and quantified, allowing for analysis of cell size, granularity, surface markers, intracellular proteins, and other key characteristics. This makes flow cytometry ideal for phenotyping immune cells, evaluating cell cycle progression, and measuring apoptosis or proliferation. Cell sorters, a type of flow cytometer with active sorting capabilities, are used for automated separation of specific cell populations based on defined parameters. This includes sorting T cells, B cells, cancer stem cells, and genetically modified populations for downstream experiments such as sequencing, culturing, or functional assays. They are especially valuable in cell therapy manufacturing, tumor microenvironment studies, and vaccine development. Flow analyzers focus on multiparameter data acquisition without physical cell separation. These instruments are indispensable for clinical immunophenotyping, microbiological monitoring, and biomarker discovery, enabling detailed analysis of surface and intracellular targets using panels of fluorescent markers. At Wasteless Bio, we offer surplus flow cytometers, including both analyzers and sorters, from industry-leading brands. Listings provide technical details such as laser configuration, detector sensitivity, throughput, number of fluorescent channels, and software compatibility. By sourcing through Wasteless Bio, labs gain access to advanced cytometry technology while supporting cost savings and sustainable reuse of high-performance lab equipment.