Electroporators/Nucleofector

Electroporators and nucleofectors are advanced laboratory instruments designed for the efficient delivery of nucleic acids and other biomolecules into living cells using carefully calibrated electrical pulses. These instruments are essential for a wide range of scientific disciplines, including molecular biology, genetic engineering, cell therapy, biotechnology, and biomedical research. The technology works by creating temporary pores in the cell membrane, allowing substances like plasmid DNA, mRNA, siRNA, proteins, or CRISPR-Cas9 complexes to enter cells without using viral vectors. Electroporation is a well-established technique used for transfecting both standard and difficult-to-transfect cell types, such as primary human cells, immune cells (e.g., T-cells), stem cells, and tumor cells. It plays a key role in workflows involving gene knockout experiments, stable and transient gene expression, protein production, and non-viral gene editing. In clinical applications, electroporation is frequently used in CAR-T cell engineering, where maintaining cell viability and transfection efficiency is crucial. Nucleofection, an enhanced electroporation method, allows for the direct delivery of nucleic acids into the nucleus, boosting transfection success in non-dividing or sensitive cells. This is particularly valuable in stem cell reprogramming, vaccine development, and regenerative medicine research. Wasteless Bio offers a range of surplus electroporators and nucleofectors from top-tier manufacturers. Each listing includes critical information such as voltage range, pulse duration, supported cell types, cuvette compatibility, and built-in protocol settings. By sourcing through Wasteless Bio, labs save on procurement costs, reduce electronic waste, and contribute to a sustainable, circular lab economy—all while accessing high-quality tools to advance cutting-edge scientific innovation.