GAD1 Polyclonal Antibody, Invitrogen, 100 μg

GAD67 Polyclonal Antibody, Invitrogen™ (Goat Polyclonal Antibody)

Product Overview:

• Host: Goat
• Type: Polyclonal, unconjugated
• Target: GAD67 (Glutamate decarboxylase 1, GAD1), 67 kDa isoform
• Immunogen: Synthetic peptide sequence (PDSPQRREKLHK) from internal region of human GAD1
• Tested Reactivity: Human; predicted to react with bovine, canine, mouse, porcine, and rat based on sequence homology
• Applications: Flow cytometry, immunocytochemistry, Western blot
• Concentration: 0.5 mg/mL
• Formulation: Liquid in TBS with 0.5% BSA and 0.02% sodium azide, pH 7.3
• Storage: -20°C, avoid freeze/thaw cycles
• Quantity:100 μg
• Purification: Ammonium sulfate precipitation
• Research Use: For research use only (RUO)

Key Biological Context:

• GAD67 and GAD65 are enzymes that catalyze the conversion of L-glutamic acid to GABA (gamma-aminobutyric acid), a major inhibitory neurotransmitter in the brain.
• GAD67 is distributed throughout the cell and responsible for basal GABA synthesis, while GAD65 is associated with synaptic vesicle membranes and is recruited during increased neurotransmission demand.
• Loss of GAD67 is lethal, while loss of GAD65 impairs GABA neurotransmission.
• Alternative splicing produces two isoforms: the predominant GAD-67 and the minor GAD-25.

Product Identifiers:

• Catalog Number: PA519065
• UniProt ID: Q99259 (GAD1).

Storage and Handling: Store at -20°C; avoid repeated freeze/thaw cycles to maintain antibody integrity.

Intended Use: Suitable for detecting GAD67 in various species for research in neuroscience, neurochemistry, and related fields

Product Code: 11337043