BugBuster Protein Extraction Reagent

Merck Millipore™ BugBuster™ 10X Protein Extraction Reagent

Application

Concentrated detergent reagent for efficient bacterial protein extraction, ideal for soluble protein recovery in recombinant protein production, enzyme studies, and proteomics workflows. Optimized for E. coli and other gram-negative bacterial cell lysis.

Product Details

• Product Code: 10579953
• Brand: Merck Millipore™ 70921-4
• Format: 250 mL concentrate (10X)
• Promotion: 497.78 GBP valid until 2025-05-18 (use code 24096)

Key Features

• Customizable formulation: Salt-free, buffer-free design enables user-defined dilution and buffer optimization
• High compatibility: Maintains protein activity by allowing pH and ionic strength adjustment
• Rapid processing: Complete cell lysis in 10-20 minutes at room temperature
• Scale flexibility: Suitable for 1 mL microcentrifuge to multi-liter bioreactor scales

Technical Specifications

• Dilution: Prepare 1X working solution with nuclease-free water or custom buffer
• Storage: Room temperature (15-25°C)
• Compatibility: Works with His-tag purification, GST-fusion systems, and activity assays

Operational Benefits

• Activity preservation: Gentle extraction maintains enzyme functionality
• Downstream-ready: Low particulate content reduces clarification requirements
• Process control: Adjust detergent concentration for specific protein solubility needs