NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat)

NEBNext^® rRNA Depletion Kit v2 (Human/Mouse/Rat)

The NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) is a refined solution for removing abundant ribosomal RNA (rRNA) before next-generation sequencing (NGS) and other RNA-based applications. By focusing on rRNA, which typically makes up 80–90% of total RNA, this kit effectively frees capacity in downstream analyses, allowing the detection of more informative, low-abundance transcripts. Incorporating second-generation improvements in reagents, probes, and protocols, it uses an RNase H-based workflow to achieve superior depletion across a wide range of input amounts (10 ng to 1 µg) and works well with both high- and low-quality RNA samples.

Key Benefits

• Optimized Depletion: Targets cytoplasmic (5S, 5.8S, 18S, 28S, human ITS, ETS) and mitochondrial (12S, 16S) rRNA for more efficient analyses.

• Broad Compatibility: Performs reliably with low- and high-quality RNA, accommodating various sample conditions.

• Flexible Input Range: Processes RNA amounts from as little as 10 ng to as much as 1 µg.

• Fast and User-Friendly: Completion in around two hours, with less than ten minutes of hands-on time.

• Multiple Formats: Available with or without RNAClean® purification beads; if beads are needed, the NEBNext rRNA Depletion Kit v2 with Sample Purification Beads (NEB #E7405) is recommended.

This improved kit ensures that highly expressed rRNA does not overwhelm critical data, enabling more accurate profiling of genes and transcripts. Whether used for random-primed cDNA synthesis, RNA-Seq, or other RNA analysis workflows, the NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) delivers consistent performance and reliable results to streamline your research.

Storage temp: -20

Expiration date: April 2025

Catalog number: E74000L