Thermo Fisher Scientific Phusion U Hot Start DNA Polymerase

Thermo Fisher Scientific Phusion U Hot Start DNA Polymerase

Description

Thermo Fisher Scientific Phusion U Hot Start DNA Polymerase is a high-fidelity enzyme designed for demanding PCR applications where accuracy and sensitivity are critical. This polymerase is engineered to bypass uracil-containing templates and incorporates a hot-start mechanism to prevent non-specific amplification and primer degradation during reaction setup. It is particularly effective for workflows involving bisulfite-converted DNA or the prevention of carry-over contamination using dUTP and Uracil-DNA Glycosylase (UNG).

Key Features

• Engineered to bypass uracil bases in the DNA template without stalling
• Hot-start technology ensures enzyme inactivity at room temperature to increase specificity
• High-fidelity performance reduces the risk of sequence errors during amplification
• Robust tolerance to common PCR inhibitors found in complex biological samples
• High processivity allows for shorter extension times compared to standard polymerases

Applications

• Amplification of bisulfite-converted DNA for methylation analysis
• Carry-over contamination control using the dUTP/UNG system
• High-fidelity PCR for cloning and sequencing applications
• Amplification of uracil-containing templates

Specifications

• Enzyme Type: High-fidelity DNA polymerase
• Hot Start Mechanism: Chemically modified or antibody-bound for room temperature stability
• Template Compatibility: DNA containing uracil (dU) and standard DNA
• Fidelity: Significantly higher than standard Taq polymerase