Fast Protein Liquid Chromatography (FPLC) systems are specialized liquid chromatography instruments designed for the purification, isolation, and analysis of proteins, peptides, and other biomolecules. Unlike traditional HPLC, FPLC systems operate at lower pressures and use aqueous buffers, making them ideal for handling delicate biological samples without denaturation. These systems are critical in biopharmaceutical development, structural biology, recombinant protein production, and antibody purification.
FPLC systems separate proteins based on properties such as size, charge, hydrophobicity, or specific affinity using columns packed with specialized resins. Common chromatography methods include size exclusion (SEC), ion exchange (IEX), affinity chromatography, and hydrophobic interaction chromatography (HIC). These workflows are used to purify monoclonal antibodies, fusion proteins, enzymes, and vaccine components under highly controlled and reproducible conditions.
Typical FPLC setups include gradient pumps, multi-wavelength UV detectors, sample injection loops, and fraction collectors, all controlled through intuitive software interfaces. Some systems support cold-room integration or built-in refrigeration to maintain protein stability. Researchers can monitor elution profiles in real time and collect purified fractions for downstream applications such as crystallization, functional assays, structural characterization, or mass spectrometry.
FPLC is essential in labs working on therapeutic biologics, cell culture optimization, and process development. It is frequently used to validate protein expression, assess purity levels, and scale up production of biologics for clinical research.
Wasteless Bio offers a wide range of pre-owned and surplus FPLC systems from leading biotech manufacturers. Each listing includes detailed specifications such as flow rate, detector wavelength, compatible columns, pressure range, and automated fractionation settings. By sourcing FPLC systems through Wasteless Bio, researchers gain access to high-quality purification technology at lower costs—while reducing waste and supporting a sustainable, circular economy within protein-based research and development.
Fast Protein Liquid Chromatography (FPLC) systems are specialized liquid chromatography instruments designed for the purification, isolation, and analysis of proteins, peptides, and other biomolecules. Unlike traditional HPLC, FPLC systems operate at lower pressures and use aqueous buffers, making t...
