Cryostat

Cryostats are precision instruments used for the sectioning of frozen biological tissues, allowing researchers and clinicians to prepare ultra-thin slices for immediate microscopic analysis. Operating at sub-zero temperatures, typically between –20°C and –30°C, cryostats preserve tissue morphology and biomolecular integrity without chemical fixation. These instruments are indispensable in frozen histology, surgical pathology, neuropathology, and rapid intraoperative diagnostics. The cryostat’s ability to deliver near-instant tissue sections makes it a vital tool during oncological surgeries, where margin assessments must be completed in real time. In research, cryostats are commonly used for sectioning tissue samples that contain delicate structures or enzyme activity that might be disrupted by standard paraffin processing. Key components of a cryostat include a refrigerated chamber, motorized or manual microtome, antivibration design, and temperature control system for both the chamber and specimen holder. Some models offer automated sectioning, UV sterilization, and integrated touchscreen interfaces for ease of use and traceability. Cryostat sections are typically used in immunofluorescence, enzyme histochemistry, and RNA in situ hybridization, making these instruments essential in molecular pathology and research that requires preserved antigenicity. At Wasteless Bio, we offer gently used and surplus cryostats from trusted industry brands. Each listing includes specifications such as section thickness range, chamber temperature, blade holder compatibility, anti-roll mechanisms, and automation features. Purchasing through Wasteless Bio allows labs and pathology departments to obtain reliable cryosectioning tools at reduced cost, while supporting sustainable lab practices by extending the life of high-value instrumentation.