ELISA Readers

ELISA readers, also known as microplate absorbance readers, are essential laboratory instruments used to detect and quantify the results of enzyme-linked immunosorbent assays (ELISAs). These readers measure changes in color intensity that occur during the assay, correlating directly with the concentration of the target molecule—whether it be an antibody, antigen, hormone, cytokine, or other biomarker. ELISA readers are widely used across clinical diagnostics, pharmaceutical development, vaccine testing, food safety, and biomedical research. In healthcare, they are critical for detecting diseases like HIV, hepatitis, COVID-19, and autoimmune disorders. In research, they are frequently used to study immune responses, inflammatory markers, and therapeutic drug levels. These systems operate by shining light through the wells of a microplate—usually 96-well or 384-well—and measuring absorbance at specific wavelengths. Common detection wavelengths include 405 nm, 450 nm, 492 nm, and 630 nm. The absorbance values are then used to calculate the concentration of the analyte using a standard curve generated from known samples. Modern ELISA readers may include multi-wavelength detection, touchscreen interfaces, pre-programmed protocols, and integration with LIMS or cloud-based data systems. Some models also support kinetic readings for time-course experiments and offer fluorescence or luminescence modules to expand their detection capabilities beyond standard colorimetric assays. At Wasteless Bio, we offer a range of surplus and pre-owned ELISA readers from trusted manufacturers, verified for quality and performance. Listings include detailed technical specifications such as wavelength range, plate format compatibility, dynamic range, read speed, and data export options. Choosing ELISA readers through Wasteless Bio enables labs to access high-quality analytical tools at lower cost—while promoting sustainable lab operations through equipment reuse and waste reduction.