Real-Time PCR systems, also known as quantitative PCR (qPCR) machines, are advanced thermal cyclers that allow for the real-time monitoring of DNA amplification using fluorescent detection. These systems are indispensable in genomics, molecular diagnostics, clinical testing, and biopharmaceutical development, enabling researchers to quantify nucleic acids with high sensitivity and specificity.
Unlike standard PCR thermocyclers, qPCR systems detect fluorescence signals emitted during each amplification cycle, allowing for quantitative analysis of DNA or RNA targets as they are amplified. This technology is widely used in gene expression analysis, mutation screening, copy number variation (CNV) detection, viral load quantification, and pathogen identification. It is also a cornerstone in COVID-19 diagnostics and infectious disease surveillance.
Modern qPCR systems support a range of fluorescent chemistries, including SYBR Green, TaqMan probes, molecular beacons, and FRET-based assays. Many systems offer multiplexing capabilities, allowing the simultaneous detection of multiple targets in a single reaction. Advanced models include features such as gradient block optimization, fast cycling modes, automated sample handling, and integration with LIMS and data analysis software.
In clinical and regulated lab settings, qPCR plays a critical role in biomarker validation, oncology diagnostics, and GMP-compliant quality control workflows. In research environments, it is used to validate gene knockdown or overexpression, screen for SNPs, and monitor transcriptional activity across experimental conditions.
Wasteless Bio offers a variety of surplus and pre-owned real-time PCR systems from industry-leading brands. Each listing includes detailed specifications such as optical detection channels, reaction volume capacity, ramp rates, throughput, and software compatibility. By sourcing qPCR systems through Wasteless Bio, laboratories can lower capital costs while contributing to sustainable science through the reuse of high-performance instrumentation.
Real-Time PCR systems, also known as quantitative PCR (qPCR) machines, are advanced thermal cyclers that allow for the real-time monitoring of DNA amplification using fluorescent detection. These systems are indispensable in genomics, molecular diagnostics, clinical testing, and biopharmaceutical development, enabling researchers to quantify nucleic acids with high sensitivity and specificity.
