UV-Vis (Ultraviolet-Visible) spectrophotometers are essential instruments used to measure the absorbance of light within the ultraviolet and visible regions of the electromagnetic spectrum (typically 190–1100 nm). These systems are a staple in laboratories across molecular biology, biochemistry, clinical diagnostics, pharmacology, and environmental science due to their reliability, versatility, and precision.
At their core, UV-Vis spectrophotometers operate by passing a beam of light through a sample and detecting the amount of light absorbed at specific wavelengths. This absorbance correlates with the concentration of analytes, making the instrument ideal for quantitative analysis. One of the most common applications is the measurement of DNA, RNA, and protein concentrations using absorbance at 260 nm, 280 nm, and 230 nm, respectively.
In molecular biology labs, UV-Vis instruments are indispensable for evaluating nucleic acid purity and concentration before PCR, qPCR, sequencing, or cloning. Protein quantification assays, such as the Bradford or BCA assay, also rely on absorbance readings. In pharmaceutical environments, they are used for drug content uniformity testing, solubility studies, and reaction monitoring. In environmental testing, UV-Vis spectrophotometry supports water quality analysis, including detection of nitrates and phosphates.
Modern UV-Vis spectrophotometers come in both single-beam and dual-beam configurations, with options for microvolume or cuvette-based sampling. Many units offer features like automated wavelength scanning, multi-wavelength analysis, temperature control, and software integration for data tracking and regulatory compliance.
At Wasteless Bio, we provide surplus UV-Vis spectrophotometers from leading brands, offering significant cost savings without compromising performance. Listings include key details such as wavelength range, path length, sample size, and detection limits. Choosing second-hand through Wasteless Bio supports sustainability while providing access to indispensable tools for accurate, real-time biomolecular analysis.
UV-Vis (Ultraviolet-Visible) spectrophotometers are essential instruments used to measure the absorbance of light within the ultraviolet and visible regions of the electromagnetic spectrum (typically 190–1100 nm). These systems are a staple in laboratories across molecular biology, biochemistry, clinical diagnostics, pharmacology, and environmental science due to their reliability, versatility, and precision.
