Autoclaves are high-pressure steam sterilization devices essential for decontaminating laboratory equipment, media, glassware, instruments, and biohazardous waste. Using saturated steam at elevated temperatures (typically 121°C or 134°C) and pressures, autoclaves effectively eliminate bacteria, viruses, fungi, spores, and other microorganisms, ensuring sterility for critical applications in microbiology, cell culture, clinical diagnostics, and pharmaceutical production.
Autoclaves operate through cycles that combine temperature, pressure, and time to achieve validated sterility. Gravity displacement autoclaves rely on steam entering the chamber and displacing air downward, suitable for basic glassware and solid instruments. Pre-vacuum (or prevac) autoclaves use vacuum pumps to remove air before steam injection, enabling faster penetration and more uniform sterilization—ideal for porous loads, wrapped instruments, and complex geometries. Benchtop autoclaves are compact units designed for smaller labs with limited space, while large-capacity floor models support high-throughput sterilization in core facilities and production environments.
Modern autoclaves feature digital controls, programmable cycles, built-in validation ports, automated door locking, cycle logging, and steam generators for consistent performance. Many include options for liquid cycles (slow exhaust to prevent boiling over), solid waste cycles, and media prep cycles, providing flexibility for diverse laboratory protocols. Sterilization indicators, biological spore tests, and temperature/pressure data logging ensure compliance with regulatory standards such as FDA, ISO, and EN certifications.
By sourcing pre-owned autoclaves from Wasteless Bio, laboratories access reliable sterilization technology at reduced costs while supporting sustainable equipment reuse. Each autoclave listing includes specifications such as chamber volume, cycle types, pressure range, temperature accuracy, validation capabilities, and safety certifications. Autoclaves are indispensable for maintaining aseptic conditions, protecting personnel, and ensuring the integrity of sterile research and production processes.
Autoclaves are high-pressure steam sterilization devices essential for decontaminating laboratory equipment, media, glassware, instruments, and biohazardous waste. Using saturated steam at elevated temperatures (typically 121°C or 134°C) and pressures, autoclaves effectively eliminate bacteria, viru...
