Life Technologies Capture select Human Albumin Affinity Matrix

Life Technologies Capture select Human Albumin Affinity Matrix

CaptureSelect™ Human Albumin Affinity Matrix is designed for highly selective purification of human albumin and albumin fusion proteins. It enables efficient one-step purification with mild elution conditions, making it ideal for pH-sensitive albumin fusion proteins.

Specifications

• Matrix: Agarose-based, aldehyde-activated

• Particle Size: 65 ± 10 μm

• Ligand Type: CaptureSelect™ HSA affinity ligand

• Binding Capacity: ~15 mg albumin/mL resin

• Flow Rate: 50–200 cm/hr (up to 2 bar)

• Elution Conditions:

  • Neutral pH: 20 mM Tris, pH 7.4 with 2 M MgCl₂, 1 M NaCl + 50% propylene glycol, or 1 M NaCl + 0.5 M L-arginine

  • Acidic pH: 0.1 M glycine, pH 3.0

• Formulation Buffer: 20% ethanol

• Storage Conditions: 2–8°C

Uses

• Protein Purification: Ideal for isolating human albumin and albumin fusion proteins.

• Biopharmaceutical Research: Supports drug development and protein engineering.

• Laboratory Applications: Used in chromatography and protein separation.

Features

• Highly Selective: Ensures efficient purification of albumin-based proteins.

• Gentle Elution Conditions: Preserves protein integrity.

• Scalable: Suitable for both small and large-volume applications.

• Animal-Origin-Free: Uses recombinant antibody fragments produced in yeast.