Product Details
- Manufacturer
- SKU
- A14524
- Expiration Date
- Shipped From
- United Kingdom
About this product
ExpiFectamine™ 293 Transfection Kit, For 1 L Culture
Overview
The ExpiFectamine™ 293 Transfection Kit is a lipid-based transient transfection reagent system designed for high-efficiency delivery of plasmid DNA into Expi293F™ human embryonic kidney cells cultured at high density in suspension. The kit combines a cationic lipid-based ExpiFectamine™ 293 Reagent with two proprietary transfection enhancers formulated to maximise protein expression yields in Expi293 Expression System workflows. The protocol parallels standard transient transfection methods while delivering enhanced performance in high-density cultures.
Highlights
- Includes ExpiFectamine™ 293 Reagent plus Transfection Enhancers 1 and 2 for boosted expression performance.
- Optimised for transient transfection of high-density Expi293F™ suspension cultures.
- Achieves protein yields 2- to 10-fold higher than many conventional transfection reagents in similar systems.
- Applicable from small to large culture volumes with scalable performance.
At-a-glance
- Kit type
- Transient transfection reagent system
- Designed for
- Expi293F™ suspension-adapted human embryonic kidney cells
- Technique
- Lipid-mediated transient transfection
- Contents
- 2.7 mL ExpiFectamine™ 293 Reagent (3×0.9 mL), 5 mL Transfection Enhancer 1, 50 mL Transfection Enhancer 2.
- Culture volume
- Sufficient for transfection of ~1 L of culture.
- Compatible samples
- Plasmid DNA with Expi293F™ cultures.
- Storage
- 2 °C – 8 °C (refrigerated).
- Condition
- NEW — Unused reagent kit
Applications
This kit is used for transient gene delivery in high-density Expi293F™ suspension cultures to support recombinant protein expression, functional protein production and other molecular biology workflows where high yields of expressed protein are desired. It is suited to research applications in protein engineering, antibody production and expression optimisation studies using standard microbiology and cell culture equipment.
Sustainability
By rehoming surplus lab products, this listing supports waste reduction and resource efficiency.
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