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Product Details

Manufacturer
SKU
E3190
Expiration Date
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United States

About this product

Promega QuantiFluor ssDNA System

Description

The Promega QuantiFluor ssDNA System is a fluorescent dye-based quantification kit designed for the sensitive and specific measurement of single-stranded DNA (ssDNA) in solution. This system provides a more accurate alternative to traditional absorbance-based methods by specifically binding to ssDNA, reducing interference from common contaminants such as proteins, free nucleotides, or double-stranded DNA. It is typically used in workflows requiring precise nucleic acid concentration measurements before downstream enzymatic reactions or sequencing.

Key Features

  • High sensitivity for low-concentration ssDNA samples
  • Specific binding to single-stranded DNA with minimal cross-reactivity
  • Fluorescence-based detection for improved accuracy over UV spectrophotometry
  • Compatible with various fluorometers and plate readers
  • Streamlined protocol for rapid sample quantification

Applications

  • Quantification of oligonucleotide primers and probes
  • Assessment of ssDNA libraries for NGS workflows
  • Monitoring of DNA denaturation processes
  • Quality control for synthetic single-stranded DNA
  • General molecular biology research requiring precise ssDNA measurement

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