Strep-Tactin™XT Buffer Set

Strep-Tactin™XT Buffer Set

Designed for the purification of recombinant Strep-tag™II and Twin-Strep-tag™ fusion proteins using Strep-Tactin™XT affinity chromatography. Also supports regeneration of the resin for repeated use.

• Product Code: 17044437
• Kit Contents:
  • 100 mL 10x Buffer W (wash buffer)
  • 25 mL 10x Buffer BXT (elution buffer with biotin)
  • 250 mL Buffer XT-R (ready-to-use regeneration buffer)
• Buffer W Composition: Tris-Cl, NaCl, EDTA, pH 8
• Buffer BXT Composition: Tris-Cl, NaCl, EDTA, 500 mM biotin, pH 8
• Buffer XT-R Composition: 3 M MgCl₂
• Form: Reagent solutions
• Concentration: Buffer W and BXT are 10x concentrated; XT-R is ready-to-use
• Storage: 2–8°C
• Stability: 12 months after shipping
• Shipping Condition: Room temperature

• Complete buffer set for wash, elution, and regeneration steps
• Biotin-based elution enables efficient recovery of tagged proteins
• Magnesium chloride regeneration buffer restores resin binding capacity
• Compatible with Strep-Tactin™XT resin only

Causes skin and eye irritation. Use protective gloves, clothing, and eye/face protection. In case of contact with skin or eyes, rinse thoroughly with water and seek medical attention if irritation persists.