BioLegend TotalSeq-B Human Universal Cocktail, V1.0

BioLegend TotalSeq-B Human Universal Cocktail, V1.0

Description

The BioLegend TotalSeq-B Human Universal Cocktail, V1.0 is a lyophilised antibody pool designed for high-parameter single-cell analysis. It enables researchers to simultaneously measure cell surface protein expression alongside transcriptome data using CITE-seq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing) or similar multi-omic workflows. By utilising oligonucleotide-conjugated antibodies, this cocktail streamlines the labelling process for human peripheral blood mononuclear cells (PBMCs) and other immune cell populations.

Key Features

• Optimised for integration with 10x Genomics Single Cell Gene Expression solutions using Feature Barcoding technology.
• Pre-titrated antibody pool ensures consistent labelling and reduces experimental variability across samples.
• Oligonucleotide-conjugated format allows for high-throughput protein detection via sequencing.
• Designed for comprehensive immunophenotyping of human immune cell subsets in a single reaction.

Applications

• Single-cell multi-omics (CITE-seq) workflows.
• Deep immunophenotyping of human PBMCs and haematological samples.
• Characterisation of heterogeneous cell populations in immunology and oncology research.
• Discovery of protein-level biomarkers in single-cell sequencing datasets.

Specifications

• Target Species: Human
• Conjugate Type: TotalSeq-B (Oligonucleotide-tagged)
• Format: Lyophilised cocktail
• Compatibility: 10x Genomics Single Cell 3' v3 or v3.1 workflows