Western Blotting Detection Reagents

Western Blotting Detection Reagents.

Western Blotting Detection Reagents are specialized solutions and substrates used to visualize and analyze protein bands transferred onto membranes following SDS-PAGE. These reagents facilitate signal generation through chemiluminescence, chromogenic, or fluorescence methods based on antibody-antigen interactions.

Specifications

• Type: Detection substrates (chemiluminescent, colorimetric, or fluorescent)
• Compatibility: Suitable for use with HRP- or AP-conjugated secondary antibodies
• Formats: Liquid reagents or kits
• Storage: Typically 2–8°C; light-sensitive for some formulations
• Sensitivity: Ranges from low nanogram to picogram levels
• Membrane Compatibility: Nitrocellulose and PVDF membranes

Uses

• Detection and quantification of specific proteins after Western blotting
• Used in molecular biology, immunology, and diagnostic research
• Ideal for high-sensitivity protein expression analysis
• Enables visualization of protein-antibody complexes using imaging systems

Product Details

• Application: Used after primary and secondary antibody incubation
• Signal Generation: Enzyme-substrate reaction (e.g., HRP with ECL substrate)
• Detection Method: Imaging by X-ray film, CCD camera, or fluorescence scanner
• Available Kits: Single