Détails du Produit
- Fabricant
- Cytiva
- Référence
- 10773577
À propos de ce produit
Western Blotting Detection Reagents.
Western Blotting Detection Reagents are specialized solutions and substrates used to visualize and analyze protein bands transferred onto membranes following SDS-PAGE. These reagents facilitate signal generation through chemiluminescence, chromogenic, or fluorescence methods based on antibody-antigen interactions.
Specifications
- Type: Detection substrates (chemiluminescent, colorimetric, or fluorescent)
- Compatibility: Suitable for use with HRP- or AP-conjugated secondary antibodies
- Formats: Liquid reagents or kits
- Storage: Typically 2–8°C; light-sensitive for some formulations
- Sensitivity: Ranges from low nanogram to picogram levels
- Membrane Compatibility: Nitrocellulose and PVDF membranes
Uses
- Detection and quantification of specific proteins after Western blotting
- Used in molecular biology, immunology, and diagnostic research
- Ideal for high-sensitivity protein expression analysis
- Enables visualization of protein-antibody complexes using imaging systems
Product Details
- Application: Used after primary and secondary antibody incubation
- Signal Generation: Enzyme-substrate reaction (e.g., HRP with ECL substrate)
- Detection Method: Imaging by X-ray film, CCD camera, or fluorescence scanner
- Available Kits: Single
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