Product Details
- Manufacturer
- SKU
- 551849
- Expiration Date
- Shipped From
- United Kingdom
About this product
BD™ ELISPOT Human IFN-γ ELISPOT Set
Overview
The BD™ ELISPOT Human IFN-γ ELISPOT Set is a research-use-only reagent set designed for enumeration of individual human cells secreting interferon-gamma (IFN-γ) using the ELISPOT (enzyme-linked immunospot) technique. Based on a sandwich immunoassay format, the set provides high-affinity capture and detection antibodies, streptavidin-horseradish peroxidase (HRP) and uncoated ELISPOT microplates sufficient for multiple assays, enabling sensitive detection of IFN-γ-producing cells in vitro. The reagents are optimised for flexibility, allowing manual coating of capture antibodies and subsequent development of coloured spots representing single secreting cells.
Highlights
- Reagents for sensitive ELISPOT detection of human IFN-γ-secreting cells in vitro
- Includes capture antibody, biotinylated detection antibody and streptavidin-HRP
- Supplied with uncoated ELISPOT plates for flexible assay setup
- Sufficient for 10 × 96-well assays with lot-specific optimisation data
At-a-glance
- Assay type
- ELISPOT (sandwich immunoassay)
- Target analyte
- Human interferon-gamma (IFN-γ)
- Includes
- Unlabeled capture antibody, biotinylated detection antibody, streptavidin-HRP, 10 uncoated ELISPOT plates, Certificate of Analysis
- Plate format
- 10 × 96-well ELISPOT plates
- Application
- Detection and enumeration of IFN-γ secreting cells
- Regulatory status
- For Research Use Only (RUO)
- Storage
- Refrigerated (~4 °C) until use
- Condition
- NEW — Unused reagent set
Applications
This ELISPOT set supports high-sensitivity analysis of cellular immune responses by enabling detection and quantification of IFN-γ secreting lymphocytes or other effector cells in human peripheral blood mononuclear cells (PBMCs) or cultured populations. Spot enumeration can be correlated with functional assays and phenotyping workflows such as flow cytometry, proliferation assays or cloning studies, making it suitable for immunology research and vaccine evaluation.
Sustainability
By rehoming surplus lab products, this listing supports waste reduction and resource efficiency.
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