Enzo Biochem AmpiGene qPCR 1-Step Probe Kit
Overview
The AmpiGene qPCR 1-Step Probe Kit is a high-performance system designed for rapid and sensitive real-time PCR directly from RNA templates. It utilizes a blended Reverse Transcriptase and a hot-start Taq polymerase to facilitate cDNA synthesis and PCR amplification in a single tube, minimizing handling and contamination risks.
Highlights
- Advanced buffer chemistry allows for high sensitivity even with low-copy number targets.
- Integrated 1-step protocol reduces experimental time by eliminating separate cDNA synthesis steps.
- Compatible with a wide range of probe-based detection chemistries, including TaqMan, Molecular Beacons, and Scorpion probes.
- Engineered to resist common PCR inhibitors often found in complex biological samples.
At-a-glance
- Capabilities
- Targeted RNA quantification; single-tube reverse transcription and qPCR.
- Form
- Kit containing AmpiGene 1-Step Mix and Enzyme Blend.
- Recommended Applications
- Gene expression analysis, RNA virus detection, and pathogen quantification.
- Compatibility
- Compatible with standard and fast-cycling real-time PCR instruments.
- Storage Condition
- -20°C
- Condition
- Surplus/second-hand; stored under manufacturer-recommended conditions.
Applications
This kit is optimized for quantitative gene expression studies and high-throughput diagnostic screening. It is frequently utilized in virology for the detection of RNA pathogens and in molecular biology for mRNA level validation across diverse cell types.
Sustainability
By rehoming surplus lab products, this listing supports waste reduction and resource efficiency.
Responsible use & safety
- Require training/authorization before use.
- Inspect before operation; do not use if safety features are compromised.
- Follow manufacturer's manual for operation and maintenance.
- Equipment-specific guidance (e.g., keep airflow clear, replace filters regularly, decontaminate after use).
