Product Details
- Manufacturer
- SKU
- ab174448
- Shipped From
- United Kingdom
About this product
Human GM-CSF ELISA Kit (CSF2)
Overview
The Human GM-CSF ELISA Kit (CSF2) is a quantitative sandwich enzyme-linked immunosorbent assay designed to measure human granulocyte-macrophage colony-stimulating factor (GM-CSF/CSF2) in biological samples such as serum, plasma and cell culture supernatants. This SimpleStep® ELISA format uses a single-wash, mix-incubate-read protocol that can be completed in approximately 90 minutes and is compatible with standard microplate readers at 450 nm. The assay leverages high-quality monoclonal antibodies to deliver specific and reproducible detection within a defined dynamic range.
Highlights
- Sandwich ELISA for quantitative detection of human GM-CSF (CSF2)
- SimpleStep® single-wash protocol for rapid workflow (~90 min)
- Compatible with standard 96-well microplate readers at 450 nm
- High sensitivity (~3.7 pg mL⁻¹) with broad dynamic range
At-a-glance
- Assay type
- Sandwich ELISA (quantitative)
- Target analyte
- Human GM-CSF/CSF2
- Sample formats
- Serum, plasma (EDTA, citrate, heparin), cell culture media/supernatant
- Detection method
- Colorimetric at 450 nm
- Sensitivity
- ~3.7 pg mL⁻¹
- Assay range
- ~15.6–1000 pg mL⁻¹
- Assay time
- ~1 h 30 min
- Format
- 96-well microplate (pre-coated)
- Condition
- NEW — Unused reagent kit
Applications
This ELISA kit is suitable for quantitative measurement of GM-CSF levels in human biological samples, supporting research in immunology, inflammation and cell signaling where GM-CSF is a key cytokine. It is used in studies of immune cell activation, cytokine profiling and translational assays that require sensitive and reproducible detection across multiple sample types.
Sustainability
By rehoming surplus lab products, this listing supports waste reduction and resource efficiency.
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