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Product Details

Manufacturer
SKU
ab174446
Shipped From
United Kingdom

About this product

Human RANTES ELISA Kit (CCL5)

by Abcam · SKU ab174446

Overview

The Human RANTES ELISA Kit (CCL5) is a quantitative sandwich enzyme-linked immunosorbent assay that uses Abcam’s SimpleStep® ELISA format to measure human RANTES (regulated on activation, normal T cell expressed and secreted; CCL5) in biological samples including serum, plasma and cell culture supernatants. The straightforward mix-wash-read workflow typically completes in approximately 90 minutes and is compatible with standard microplate readers at 450 nm. This kit employs high-quality antibodies for specific, reproducible detection within a defined dynamic range.

Highlights

  • Sandwich ELISA for quantitative detection of human RANTES/CCL5
  • SimpleStep® single-wash protocol with ~90 min assay time
  • High sensitivity down to ~0.091 pg mL⁻¹
  • Compatible with standard 450 nm microplate readers

At-a-glance

Assay type
Sandwich ELISA (quantitative)
Target analyte
Human RANTES (CCL5)
Sample format
Pre-coated 96-well microplate
Suitable samples
Serum, plasma (EDTA, citrate, heparin), cell culture supernatants
Detection method
Colorimetric at 450 nm
Sensitivity
~0.091 pg mL⁻¹
Assay range
~0.94–60 pg mL⁻¹
Assay time
~1 h 30 min
Condition
NEW — Unused reagent kit

Applications

This kit is suitable for quantitative measurement of human RANTES/CCL5 concentrations in clinical research and cell biology applications, including immunology and inflammation studies. It supports analysis of cytokine profiles in serum, plasma and conditioned media where RANTES is a biomarker of interest. Standard microplate readers are used to quantify the chromogenic signal proportional to analyte concentration.

Sustainability

By rehoming surplus lab products, this listing supports waste reduction and resource efficiency.

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