IsoFast Bst Polymerase

IsoFast Bst Polymerase

Description:

IsoFast® Bst Polymerase is a DNA polymerase with significant strand displacement activity, making it ideal for various nucleic acid amplification techniques. It is particularly well-suited for whole genome amplification, multiple displacement amplification, and isothermal amplification methods. The enzyme is a recombinant form of the large fragment of Bst DNA polymerase, engineered to retain 5'-3' polymerase activity while lacking 5'-3' exonuclease activity. It performs optimally at 65°C but demonstrates functionality across a broad temperature range (55°C-70°C) and is inactivated at 80°C.

The IsoFast® Hot Start Bst Polymerase & Mixes variant integrates AptaLock™ hot start technology, ensuring superior specificity and sensitivity for isothermal amplification workflows, LAMP applications, and point-of-care testing scenarios. This version minimizes primer dimer formation and non-specific target amplification.

Key Features:

• Robust strand displacement capability
• Efficient for isothermal amplification processes
• AptaLock™ hot start technology for enhanced precision (Hot Start variant)
• Versatile format options, including ready-to-use mixes and fluorescent dye integration

Applications:

• Whole genome amplification
• Multiple displacement amplification
• Isothermal amplification
• Loop-mediated isothermal amplification (LAMP)
• Molecular diagnostics
• Point-of-care testing

Technical Specifications:

• Enzyme Type: DNA polymerase
• Key Activity: Strand displacement
• Optimal Temperature: 65°C (functional range: 55°C-70°C)
• Heat Inactivation: 80°C
• Exonuclease Activity: None (5'-3')
• Total Reaction Volume: 25 µl
• Storage Temperature: -20°C

Manufacturer: PCR Biosystems