Product Details
- Manufacturer
- SKU
- DY994
- Expiration Date
- Shipped From
- United Kingdom
About this product
Stop Solution 2N Sulfuric Acid, 6 mL
Overview
Stop Solution 2N Sulfuric Acid (Catalog # DY994) from R&D Systems is a ready-to-use acidic reagent formulated to halt the enzymatic activity of horseradish peroxidase (HRP) in enzyme-linked immunosorbent assays that use tetramethylbenzidine (TMB) substrates. Each vial contains 6 mL of 2 N sulfuric acid, with typical application volumes of 50–100 µL per well to terminate colour development before optical density measurement. The product is supplied as multiple vials per pack and designed for consistent colour stabilisation in ELISA workflows.
Highlights
- 2 N sulfuric acid solution to stop HRP-catalysed TMB reactions in ELISAs
- Pre-measured 6 mL aliquots for reproducible use across microplate wells
- Facilitates stable endpoint colour for accurate absorbance reading at ~450 nm
- Suitable for a wide range of research immunoassays with HRP detection
At-a-glance
- Reagent
- 2 N sulfuric acid stop solution
- Volume
- 6 mL per vial
- Application
- Termination of HRP-TMB reactions in ELISA assays
- Typical use
- ~50–100 µL per well
- Pack configuration
- 15 vials × 6 mL (per standard listing)
- Condition
- NEW — Unused reagent
Applications
This stop solution is used to quench the enzymatic conversion of TMB by horseradish peroxidase in ELISAs and related immunoassays, stabilizing the colorimetric signal for quantification with standard microplate readers at ~450 nm. It supports research workflows in cytokine, growth factor, antibody and biomarker quantification where accurate endpoint detection is essential.
Sustainability
By rehoming surplus lab products, this listing supports waste reduction and resource efficiency.
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