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Product Details

Manufacturer
Cytiva
SKU
10773577

About this product

Western Blotting Detection Reagents.

Western Blotting Detection Reagents are specialized solutions and substrates used to visualize and analyze protein bands transferred onto membranes following SDS-PAGE. These reagents facilitate signal generation through chemiluminescence, chromogenic, or fluorescence methods based on antibody-antigen interactions.

Specifications

  • Type: Detection substrates (chemiluminescent, colorimetric, or fluorescent)
  • Compatibility: Suitable for use with HRP- or AP-conjugated secondary antibodies
  • Formats: Liquid reagents or kits
  • Storage: Typically 2–8°C; light-sensitive for some formulations
  • Sensitivity: Ranges from low nanogram to picogram levels
  • Membrane Compatibility: Nitrocellulose and PVDF membranes

Uses

  • Detection and quantification of specific proteins after Western blotting
  • Used in molecular biology, immunology, and diagnostic research
  • Ideal for high-sensitivity protein expression analysis
  • Enables visualization of protein-antibody complexes using imaging systems

Product Details

  • Application: Used after primary and secondary antibody incubation
  • Signal Generation: Enzyme-substrate reaction (e.g., HRP with ECL substrate)
  • Detection Method: Imaging by X-ray film, CCD camera, or fluorescence scanner
  • Available Kits: Single

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RNAnalytics

RNAnalytics

Leading The Way In Gene Therapy Analytics

As a startup focused on developing analytical toolkits for LNP characterization, RNAnalytics operates under tight budget constraints while striving to maintain a fully stocked lab with high-quality materials. Sustainability is also a core pillar of our operations, pushing us to make environmentally conscious decisions wherever possible....