NEBNext® Poly(A) mRNA Magnetic Isolation Module

NEBNext^® Poly(A) mRNA Magnetic Isolation Module

The module uses Oligo d(T)25 coupled to 1 μm paramagnetic beads, allowing direct binding of poly(A)+ RNA from 1–5 µg of total RNA. This technique enables easy manual processing or seamless integration into automated high-throughput workflows. Magnetic separation technology eliminates the need for precipitation steps, providing intact mRNA in small elution volumes.

It offers a fast and reliable solution for isolating intact poly(A)+ RNA from total RNA samples. This streamlined approach ensures efficient removal of ribosomal RNA (rRNA), which typically constitutes 80–90% of total RNA, making it ideal for cost-effective sequencing. The isolation process takes less than one hour, saving time while maintaining high-quality results.

• Properties & Usage

  Materials Required but not Supplied

  • 96-well 0.2 ml PCR Plates and Microseal® 'B' Adhesive Sealer (BioRad MSB-1001) or 0.2 ml RNase-free tube.
  • Magnetic rack or plate (e.g., NEBNext^® Magnetic Separation Rack (NEB #S1515S), Alpaqua ^® 96S Super Magnet Plate (#A001322), or equivalent)
  • Thermal cycler or heat block
  • Bioanalyzer^® (Agilent Technologies, Inc.) or similar instrument and consumables.
• Application Features

  Isolation of poly(A)+ RNA transcript from Total RNA for RNA library preparation and sequencing.

  Catalog Number: E7490l

  Expiration Date: October 2025