Thermo Orbitrap Velos Pro Hybrid Mass Spectrometer
Overview
The Thermo Orbitrap Velos Pro is a high-performance hybrid mass spectrometer combining a dual-pressure linear ion trap with an Orbitrap mass analyzer. It delivers high resolution, accurate mass data and multi-stage MSn capabilities for comprehensive analysis of complex samples across proteomics, metabolomics and structural characterisation. This configuration includes ion sources, vacuum pumps, chiller unit, acquisition computer with installed software and accessories supplied by the seller.
Highlights
- Hybrid ion trap–Orbitrap architecture with dual-pressure linear ion trap front end
- High resolution and accurate mass measurements suitable for complex mixture analysis
- Multiple fragmentation modes (CID, HCD and optional ETD) for structural elucidation
- Parallel MS and multi-stage MSn acquisition for enhanced analytical depth
At-a-glance
- Condition
- Used — Unit powered off in fully functional condition as of March 2024 with calibration and cleaning certificates dated July 23, 2023
- Technology
- Hybrid Orbitrap mass analyser with Velos Pro dual-pressure linear ion trap front end
- Mass analyser type
- Orbitrap high-resolution accurate mass (HR/AM) and linear ion trap MSn
- Resolving power
- Up to ~100 000 FWHM at m/z 400 (Orbitrap) with high-accuracy mass measurements
- Mass range
- m/z 50–2000 typical; extended up to m/z 4000 in some modes
- Fragmentation
- CID, HCD; optional ETD available
- Scan functions
- Full-scan, MS/MS and MSn for n≥1 capabilities
- Ionisation compatibility
- API sources including ESI/HESI with supplemental gas options possible (dependent on installed source)
- Included components
- Primary mass spectrometer, ionisation source, two oil-free vacuum pumps, chiller, acquisition computer (software installed), monitor, keyboard, mouse, network cables, clamps, tubing, spray monitors, minor accessories
Applications
This hybrid mass spectrometer is applied extensively in high-resolution proteomics and metabolomics studies, enabling confident peptide and protein identification in complex mixtures. The combination of accurate mass measurement, multiple fragmentation regimes and MSn supports detailed structural elucidation of metabolites, post-translational modifications, and low-abundance analytes. It is also utilised in forensic and environmental research for comprehensive compound profiling.
