Cytiva HiTrap Capto PlasmidSelect

Cytiva HiTrap Capto PlasmidSelect

Description

Cytiva HiTrap Capto PlasmidSelect columns are designed for the purification of supercoiled plasmid DNA for research and clinical applications. These prepacked affinity chromatography columns utilise a thiophilic aromatic adsorption resin that specifically recognises the conformation of supercoiled DNA, allowing for the effective separation of the active supercoiled form from open-circular isoforms and other impurities such as host cell proteins and RNA.

Key Features

• High selectivity for supercoiled plasmid DNA over other DNA isoforms and impurities.
• Prepacked format ensures reproducible results and ease of use in laboratory workflows.
• Compatible with standard chromatography systems and manual syringe operation.
• Rigid resin matrix allows for high flow rates and efficient processing times.
• Scalable technology suitable for transitioning from small-scale research to larger production.

Applications

• Purification of supercoiled plasmid DNA for gene therapy and vaccine research.
• Removal of open-circular DNA and genomic DNA contaminants.
• Downstream processing in molecular biology and biotechnology laboratories.
• Preparation of high-purity DNA for transfection and transformation protocols.

Specifications

• Ligand: Thiophilic aromatic adsorption
• Matrix: Highly cross-linked agarose
• pH Stability (Working): 3 to 12
• pH Stability (Cleaning-in-place): 2 to 14
• Operating Pressure Max: 3 bar (0.3 MPa)
• Recommended Flow Rate: 1 ml/min

Disclaimer

These columns are intended for laboratory research and process development use. Users should verify compatibility with specific buffer systems and target plasmid sizes before use.