Product Details
- Manufacturer
- SKU
- ab176109
- Shipped From
- United Kingdom
About this product
Human SCF ELISA Kit
Overview
The Human SCF ELISA Kit (ab176109) from Abcam is a quantitative sandwich enzyme-linked immunosorbent assay designed to measure human stem cell factor (SCF) concentrations in biological samples including plasma, serum and cell culture media. This SimpleStep® ELISA format uses a single-wash, mix-incubate-read protocol, enabling assay completion in approximately 90 minutes with a standard microplate reader at 450 nm. The kit employs high-quality monoclonal capture and detection antibodies to facilitate specific and reproducible SCF quantification across the declared dynamic range.
Highlights
- Sandwich ELISA for quantitative detection of human stem cell factor (SCF)
- SimpleStep® single-wash protocol for rapid workflow (~90 min)
- Compatible with standard 96-well microplate readers at 450 nm
- Sensitivity down to ~0.51 pg mL⁻¹ with broad dynamic range
At-a-glance
- Assay type
- Sandwich ELISA (quantitative)
- Analyte
- Human stem cell factor (SCF), also known as KITLG/Kit ligand
- Sample format
- 96-well microplate
- Suitable samples
- Plasma, serum, cell culture media
- Detection method
- Colorimetric, 450 nm
- Sensitivity
- ~0.51 pg mL⁻¹
- Assay range
- ~15.6 – 1000 pg mL⁻¹
- Assay time
- ~1 h 30 min
- Condition
- NEW — Unused reagent kit
Applications
This ELISA kit is suitable for quantitative measurement of SCF levels in human clinical research samples including serum and plasma, as well as cell culture supernatants in basic and translational studies. It supports studies of hematopoiesis, stem cell biology and related signalling pathways where SCF concentration is a variable of interest. Standard microplate instrumentation is sufficient for read-out, facilitating integration into existing immunoassay workflows.
Sustainability
By rehoming surplus lab products, this listing supports waste reduction and resource efficiency.
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