IsoFast Bst 1-Step Mix

IsoFast Bst 1-Step Mix

Application: IsoFast® Bst 1-Step Mix is a versatile isothermal amplification reagent designed for rapid and sensitive one-step reverse transcription and DNA amplification of RNA targets. It combines strand-displacing IsoFast Bst DNA Polymerase with a highly active modified MMLV Reverse Transcriptase (RTase Go) in a 2x master mix format, enabling efficient nucleic acid amplification at a constant temperature of 65°C.

The product line includes multiple pack sizes and formats suitable for real-time or end-point detection in molecular diagnostics, infectious disease detection, and research applications such as RT-LAMP.

Product Details:

• Dual enzyme system: IsoFast Bst strand-displacing polymerase and modified MMLV RTase
• 2x master mix with integrated enzymes, buffer, fluorescent dye, and RNase inhibitor
• Isothermal amplification protocol at 65°C, typically completed in 30 minutes
• Fluorescence-based real-time detection compatibility
• Available pack sizes commonly range from 100 to 500 reactions (25 µL each)
• Optimized for robust performance across diverse nucleic acid templates and sample types
• Validated for SARS-CoV-2 detection and other viral RNA targets
• Storage temperature: -20°C
• Manufacturer: PCR Biosystems

Key Features:

• Rapid and highly sensitive detection of RNA targets in a single step
• Enhanced strand displacement for efficient amplification
• Robust amplification even in presence of inhibitors
• Compatible with a variety of real-time PCR and isothermal platforms
• Reliable enzyme mix designed for routine molecular biology and diagnostic use