- Quality focus
- Sustainable sourcing
- Supplier accountability
- Industry trust
We partner with reputable suppliers who have vested interest to uphold their brand integrity, prioritize sustainability by reducing waste, and maintain transparency in product listings. As a B2B marketplace, we rely on supplier accountability while encouraging best practices, though final quality and compliance remain their responsibility.
Product Details
- Manufacturer
- SKU
- GCM07.2500
- Expiration Date
- Shipped From
- Portugal
About this product
Terrific Broth
Application: Nutritionally rich liquid medium designed for the growth and maintenance of recombinant Escherichia coli strains in molecular biology, supporting high cell density and extended logarithmic phase cultivation.
Product Details:
- Dehydrated powder
- Appearance: Beige powder; amber-colored when prepared and autoclaved
- Storage: 2ºC to 25ºC; keep container tightly closed
- Preparation: Dissolve 47.6 g powder in 900 mL distilled water, add 4 mL glycerol, adjust volume to 1 L, heat to dissolve, autoclave at 121ºC for 15–20 min, store at 2ºC to 8ºC
- Formulation (g/L): Tryptone 12.00, Yeast Extract 24.00, K2HPO4 12.54, KH2PO4 2.31
- Final pH: 7.2 ± 0.2 at 25ºC
Key Features:
- Supports robust high-density growth of recombinant E. coli
- Phosphate buffer prevents acidification and supports cell viability
- Glycerol as carbon source avoids acetic acid buildup and glucose repression
- Rich mix of nutrients including carbon, nitrogen, vitamins, and minerals
- Compatible with addition of heat-sensitive additives post-sterilization
Applications:
- Plasmid DNA production at high yield
- Recombinant protein expression studies
- Prolonged logarithmic phase cultivation
- Molecular and biotechnological research
Related Products
What Our Clients Say
Supporting innovation at leading research companies
RNAnalytics
Leading The Way In Gene Therapy Analytics
As a startup focused on developing analytical toolkits for LNP characterization, RNAnalytics operates under tight budget constraints while striving to maintain a fully stocked lab with high-quality materials. Sustainability is also a core pillar of our operations, pushing us to make environmentally conscious decisions wherever possible....