Perfusion Systems

Perfusion systems are advanced bioprocessing platforms designed to support continuous cell culture by enabling the steady exchange of fresh media while simultaneously removing waste and byproducts from the bioreactor. Widely used in biopharmaceutical manufacturing, vaccine development, cell therapy, and long-term culture workflows, these systems enhance cell viability, productivity, and consistency in both small-scale research and large-scale GMP-compliant production environments. Unlike traditional batch or fed-batch processes, perfusion culture maintains optimal conditions for cell growth over extended periods by supplying nutrients continuously and keeping metabolite accumulation to a minimum. This allows cells—particularly fragile or high-density suspension cultures—to remain in an exponential growth phase for longer, significantly boosting product yields and process efficiency. As a result, perfusion systems are increasingly employed for monoclonal antibody (mAb) production, recombinant protein expression, and viral vector manufacturing. Modern perfusion systems are typically integrated with bioreactors, hollow fiber filters, alternating tangential flow (ATF) devices, or tangential flow filtration (TFF) modules, which separate cells from spent media while retaining them in the culture vessel. These closed-loop designs support sterile, scalable, and automated operations and are compatible with single-use bioreactors, enabling rapid changeover and simplified cleaning validation. Additional components such as cell retention devices, sterile connectors, sensors, and automated pumps allow for precise control of flow rates, cell density, and process parameters. Many perfusion systems also integrate with Process Analytical Technology (PAT) platforms to support real-time monitoring and optimization of key variables such as pH, dissolved oxygen, glucose concentration, and cell viability. At Wasteless Bio, we offer surplus and refurbished perfusion systems and components from industry-leading suppliers. Each listing includes specifications such as flow rate capacity, compatible bioreactor volume, filtration method, material compatibility, and GMP-readiness. By sourcing perfusion systems through Wasteless Bio, biomanufacturers and researchers can build high-performance, cost-effective cell culture workflows while advancing sustainable, closed-system processing practices.