ZYMO Research Zyppy Plasmid Miniprep Kit

ZYMO Research Zyppy Plasmid Miniprep Kit

Description

The Zyppy Plasmid Miniprep Kit isolates high-quality plasmid DNA from overnight Escherichia coli cultures for routine molecular biology workflows. Its pellet-free preparation streamlines sample handling by lysing the culture directly before purification on a Zymo-Spin column.

Key Features

• Pellet-free modified alkaline lysis avoids conventional cell pelleting and resuspension.
• Direct culture processing uses 7X Lysis Buffer, neutralisation and Zymo-Spin column purification.
• Coloured buffers help visualise complete cell lysis and neutralisation.
• The preparation includes an Endo-Wash step and produces endotoxin-free plasmid DNA suitable for sensitive downstream work.

Applications

• PCR, DNA sequencing and cloning.
• DNA ligation, restriction endonuclease digestion and in vitro transcription.
• Bacterial transformation and transfection of eukaryotic cell lines.

Specifications

• Starting material: 600 µl of overnight Escherichia coli culture.
• Equipment: microcentrifuge and/or vacuum manifold.
• Processing time: 8 minutes.
• Elution volume: ≥30 µl per preparation reference volume for the kit workflow discussed on the product page; confirm protocol requirements before use.