Sartorius Incucyte Cytolight Rapid Dye

Sartorius Incucyte Cytolight Rapid Dye

Description

Sartorius Incucyte Cytolight Rapid Dye is a fluorescent reagent for rapid cytoplasmic labeling of living cells in live-cell assays.

Key Features

• After entering cells, the dye is transformed into cell-impermeant fluorescent probes that are transferred by dilution to daughter cells rather than adjacent cells.
• At optimized concentrations, labeling is non-perturbing to growth and morphology in healthy cells and remains well-retained in cells.
• Suitable for cell types that are difficult to label using BacMam, lentivirus or other transfection-based approaches.
• Validated for use with the Incucyte Live-Cell Analysis System and integrated software.

Applications

• Identifying labeled cell locations and monitoring dynamic interactions in mixed cultures.
• Immune-cell killing assays, including cytotoxic T-cell killing, antibody-dependent cell-mediated cytotoxicity and immune-cell killing in tumor spheroid models.
• Combining live-cell labeling with Incucyte Cell Health Reagents for simultaneous apoptosis or cytotoxicity readouts.

Specifications

• Form: Dry powder.
• Labeling capacity: 10–90 million cells, depending on optimized concentration.
• Storage: Store dry powder desiccated at -20 °C; after solubilization with DMSO, store the solution at -20 °C and protect it from light.

Disclaimer

Signal intensity decreases as fluorescence is diluted between daughter cells; measurements beyond 48 hours post-labeling are generally not recommended. Long-term proliferation measurements in fast-growing cells are also not recommended because declining fluorescence can impair fluorescence area and count metrics.