Sartorius Incucyte Rat Fabfluor-pH Antibody Labeling Dye for Antibody Internalization

Sartorius Incucyte Rat Fabfluor-pH Antibody Labeling Dye for Antibody Internalization

Description

Incucyte Rat Fabfluor-pH Red Antibody Labeling Dye is a pH-sensitive, dye-coupled antibody fragment for labeling Fc-containing rat test antibodies. Added directly to tissue-culture wells using a no-wash, mix-and-read workflow, it enables live-cell imaging of adherent and non-adherent cells. Internalized labeled antibodies generate a fluorogenic signal during processing through acidic lysosomes and endosomes, allowing antibody uptake to be monitored and quantified with Incucyte integrated software.

Key Features

• Labels Fc-containing rat test antibodies for antibody internalization studies.
• Uses a pH-sensitive fluorogenic response during intracellular processing.
• Supports direct addition to tissue-culture wells without a wash step.
• Compatible with live-cell imaging of adherent and non-adherent cells.
• Compatible with Incucyte SX1, S3 and SX5 instruments.

Applications

• Live-cell antibody internalization assays.
• Cell-based labeling and antibody uptake studies.
• Kinetic comparison of antibody uptake across candidates.
• High-throughput antibody selection and optimization studies for biologics drug discovery.

Specifications

• Species/isotype: Rat; target: Rat.
• Assay type: Antibody labeling.
• Research area: Cell function research; application: Antibody internalization when used as intended by the assay designer.